Review



anti-mouse cyclin-dependent kinase 6 (cdk6) antibody  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Proteintech anti-mouse cyclin-dependent kinase 6 (cdk6) antibody
    Anti Mouse Cyclin Dependent Kinase 6 (Cdk6) Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-mouse cyclin-dependent kinase 6 (cdk6) antibody/product/Proteintech
    Average 90 stars, based on 1 article reviews
    anti-mouse cyclin-dependent kinase 6 (cdk6) antibody - by Bioz Stars, 2026-02
    90/100 stars

    Images



    Similar Products

    anti cdk6 antibody  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti cdk6 antibody
    Anti Cdk6 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cdk6 antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    anti cdk6 antibody - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    anticdk6 antibody  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anticdk6 antibody
    Anticdk6 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anticdk6 antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    anticdk6 antibody - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    anti-mouse cyclin-dependent kinase 6 (cdk6) antibody  (Proteintech)
    90
    Proteintech anti-mouse cyclin-dependent kinase 6 (cdk6) antibody
    Anti Mouse Cyclin Dependent Kinase 6 (Cdk6) Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-mouse cyclin-dependent kinase 6 (cdk6) antibody/product/Proteintech
    Average 90 stars, based on 1 article reviews
    anti-mouse cyclin-dependent kinase 6 (cdk6) antibody - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    antibody cdk6 mouse monoclonal cell signaling  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc antibody cdk6 mouse monoclonal cell signaling
    Figure 4. CDK4/6 interactions and conformations. (A) Illustration of regulatory CDK4/6 interactions and Rb activation. (B) Domain organization of CDK4, <t>CDK6,</t> and p16INK4a; tested point mutations are listed. (C) 3D structure of CDK6 in complex with p16INK4a. Crucial amino acids involved in the interaction of the two proteins are highlighted. The R31C mutant is depicted in orange (PDB code: 1BI7, Russo et al., 1998). (D) Protein:protein interaction (PPI) reporter analyses of the kinases CDK4 and CDK6 with p16INK4a. Scheme illustrates CDK4/6 hetero-dimer formation with p16INK4a analyzed using a PCA RLuc PPI reporter system. PPI induces the complementation of RLuc PCA fragments promoting an increase in bioluminescence (HEK293T cells, 48 hr of transient reporter expression). Bars represent the RLU fold change of PPI in relation to wild-type CDK4/6:p16INK4a complex (mean ± SEM, n=7 ind. experiments). (E) Basal signal of CDK4/6 KinCon reporters with indicated mutations are shown (expressed for 48 hr in HEK293T cells) (mean
    Antibody Cdk6 Mouse Monoclonal Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody cdk6 mouse monoclonal cell signaling/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    antibody cdk6 mouse monoclonal cell signaling - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    cdk6 mouse monoclonal antibody  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc cdk6 mouse monoclonal antibody
    Figure 4. CDK4/6 interactions and conformations. (A) Illustration of regulatory CDK4/6 interactions and Rb activation. (B) Domain organization of CDK4, <t>CDK6,</t> and p16INK4a; tested point mutations are listed. (C) 3D structure of CDK6 in complex with p16INK4a. Crucial amino acids involved in the interaction of the two proteins are highlighted. The R31C mutant is depicted in orange (PDB code: 1BI7, Russo et al., 1998). (D) Protein:protein interaction (PPI) reporter analyses of the kinases CDK4 and CDK6 with p16INK4a. Scheme illustrates CDK4/6 hetero-dimer formation with p16INK4a analyzed using a PCA RLuc PPI reporter system. PPI induces the complementation of RLuc PCA fragments promoting an increase in bioluminescence (HEK293T cells, 48 hr of transient reporter expression). Bars represent the RLU fold change of PPI in relation to wild-type CDK4/6:p16INK4a complex (mean ± SEM, n=7 ind. experiments). (E) Basal signal of CDK4/6 KinCon reporters with indicated mutations are shown (expressed for 48 hr in HEK293T cells) (mean
    Cdk6 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdk6 mouse monoclonal antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    cdk6 mouse monoclonal antibody - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    mouse cdk6 monoclonal antibody  (Proteintech)
    96
    Proteintech mouse cdk6 monoclonal antibody
    Figure 3. Effects of RAPA on the cell cycle progress of B16‑F10 cells. (A) Cell cycle distribution was assessed by flow cytometry. (B) Results of statistical analysis of cell rates at different time periods for each group in (A). (C) CDK1, cyclin D1, CDK4, <t>CDK6,</t> cyclin E1 and CDK2 protein expression levels were detected by western blotting. Relative expression of (D) CDK1, (E) Cyclin D1, (F) CDK4, (G) CDK6, (H) Cyclin E1 and (I) CDK2 proteins. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. RAPA 0 nM group. ns, not significant; RAPA, rapamycin.
    Mouse Cdk6 Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse cdk6 monoclonal antibody/product/Proteintech
    Average 96 stars, based on 1 article reviews
    mouse cdk6 monoclonal antibody - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    resource source identifier antibodies cdk6 ab cell signaling  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc resource source identifier antibodies cdk6 ab cell signaling
    Figure 3. Effects of RAPA on the cell cycle progress of B16‑F10 cells. (A) Cell cycle distribution was assessed by flow cytometry. (B) Results of statistical analysis of cell rates at different time periods for each group in (A). (C) CDK1, cyclin D1, CDK4, <t>CDK6,</t> cyclin E1 and CDK2 protein expression levels were detected by western blotting. Relative expression of (D) CDK1, (E) Cyclin D1, (F) CDK4, (G) CDK6, (H) Cyclin E1 and (I) CDK2 proteins. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. RAPA 0 nM group. ns, not significant; RAPA, rapamycin.
    Resource Source Identifier Antibodies Cdk6 Ab Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource source identifier antibodies cdk6 ab cell signaling/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    resource source identifier antibodies cdk6 ab cell signaling - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    mouse anti cdk6  (Santa Cruz Biotechnology)
    97
    Santa Cruz Biotechnology mouse anti cdk6
    Figure 3. Effects of RAPA on the cell cycle progress of B16‑F10 cells. (A) Cell cycle distribution was assessed by flow cytometry. (B) Results of statistical analysis of cell rates at different time periods for each group in (A). (C) CDK1, cyclin D1, CDK4, <t>CDK6,</t> cyclin E1 and CDK2 protein expression levels were detected by western blotting. Relative expression of (D) CDK1, (E) Cyclin D1, (F) CDK4, (G) CDK6, (H) Cyclin E1 and (I) CDK2 proteins. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. RAPA 0 nM group. ns, not significant; RAPA, rapamycin.
    Mouse Anti Cdk6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti cdk6/product/Santa Cruz Biotechnology
    Average 97 stars, based on 1 article reviews
    mouse anti cdk6 - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 4. CDK4/6 interactions and conformations. (A) Illustration of regulatory CDK4/6 interactions and Rb activation. (B) Domain organization of CDK4, CDK6, and p16INK4a; tested point mutations are listed. (C) 3D structure of CDK6 in complex with p16INK4a. Crucial amino acids involved in the interaction of the two proteins are highlighted. The R31C mutant is depicted in orange (PDB code: 1BI7, Russo et al., 1998). (D) Protein:protein interaction (PPI) reporter analyses of the kinases CDK4 and CDK6 with p16INK4a. Scheme illustrates CDK4/6 hetero-dimer formation with p16INK4a analyzed using a PCA RLuc PPI reporter system. PPI induces the complementation of RLuc PCA fragments promoting an increase in bioluminescence (HEK293T cells, 48 hr of transient reporter expression). Bars represent the RLU fold change of PPI in relation to wild-type CDK4/6:p16INK4a complex (mean ± SEM, n=7 ind. experiments). (E) Basal signal of CDK4/6 KinCon reporters with indicated mutations are shown (expressed for 48 hr in HEK293T cells) (mean

    Journal: eLife

    Article Title: Kinases in motion: impact of protein and small molecule interactions on kinase conformations

    doi: 10.7554/elife.94755

    Figure Lengend Snippet: Figure 4. CDK4/6 interactions and conformations. (A) Illustration of regulatory CDK4/6 interactions and Rb activation. (B) Domain organization of CDK4, CDK6, and p16INK4a; tested point mutations are listed. (C) 3D structure of CDK6 in complex with p16INK4a. Crucial amino acids involved in the interaction of the two proteins are highlighted. The R31C mutant is depicted in orange (PDB code: 1BI7, Russo et al., 1998). (D) Protein:protein interaction (PPI) reporter analyses of the kinases CDK4 and CDK6 with p16INK4a. Scheme illustrates CDK4/6 hetero-dimer formation with p16INK4a analyzed using a PCA RLuc PPI reporter system. PPI induces the complementation of RLuc PCA fragments promoting an increase in bioluminescence (HEK293T cells, 48 hr of transient reporter expression). Bars represent the RLU fold change of PPI in relation to wild-type CDK4/6:p16INK4a complex (mean ± SEM, n=7 ind. experiments). (E) Basal signal of CDK4/6 KinCon reporters with indicated mutations are shown (expressed for 48 hr in HEK293T cells) (mean

    Article Snippet: DOI: https://doi.org/10.7554/eLife.94755 20 of 31 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Transfected construct mouse pcDNA3.1CDK6- F[1] CDK6- PPI reporter Transfected construct mouse pcDNA3.1CDK6- R31C- F[1] CDK6- PPI reporter (R31C) Transfected construct mouse pcDNA3.1CDK4- F[1] CDK4- PPI reporter Transfected construct mouse pcDNA3.1CDK4- R24C- F[1] CDK4- PPI reporter (R24C) Transfected construct mouse pcDNA3.1p16INK4a- F[2] p16INK4a- PPI reporter Transfected construct mouse pcDNA3.1- p16INK4a- P40L- F[2] p16INK4a- PPI reporter (P40L) Transfected construct human pcDNA3.1F[1]-MEK1- F[2] MEK1- KinCon Transfected construct human pcDNA3.1- F[1]-MEK1- K57E- F[2] MEK- KinCon (K57E) Transfected construct human pcDNA3.1F[1]-PKAc- F[2] PKAc- KinCon Transfected construct human pcDNA3.1F[1]-PKAc- L206R- F[2] PKAc- KinCon (L206R) Transfected construct human pcDNA3.1MO25- 3xFlag MO25- 3xFlag Transfected construct human pcDNA3.1F[1]-BRAF- F[2] BRAF- KinCon Transfected construct human pcDNA3.1F[1]-BRAF- V600E- F[2] BRAF- KinCon (V600E) Transfected construct human pcDNA3.1F[1]-RIPK1- F[2] RIPK1- KinCon Transfected construct human pcDNA3.1F[1]-RIPK2- F[2] RIPK2- KinCon Transfected construct human pcDNA3.1F[1]-RIPK3- F[2] RIPK3- KinCon Antibody GAPDH Rabbit monoclonal Cell Signaling 2118 1:10000 Antibody AMPKα Rabbit monoclonal Cell Signaling 2532 1:1000 Antibody PhosphoAMPKα(Thr172) Rabbit monoclonal Cell Signaling 2535 1:1000 Antibody LKB1 Rabbit monoclonal Cell Signaling 3047 1:1000 Antibody Vinculin Rabbit monoclonal Cell Signaling 4650 1:1000 Antibody RIP XP Rabbit monoclonal Cell Signaling 3493 1:1000 Antibody RIP3 Rabbit monoclonal Cell Signaling 13526 1:1000 Antibody CDK6 Mouse monoclonal Cell Signaling 3136 1:1000 Continued Continued on next page Kugler, Schwaighofer et al. eLife 2024;13:RP94755.

    Techniques: Activation Assay, Mutagenesis, Expressing

    Figure 5. Impact of small molecules and protein interactions on kinase activity conformations. (A+B) Depiction of molecular interactions of a type I 1/2 and type III kinase inhibitors with a kinase domain (N and C lobe). Impact of PLX8394, Cobimetinib and GSK547 on wild-type (wt) and mutated versions of BRAF, RIPK1, and MEK1 kinase conformation (KinCon) reporters. 48 hr post transfection HEK293T cells expressing respective reporter constructs were treated with indicated inhibitors for 1 hr (1 μM) followed by RLuc PCA analyses (mean ± SEM, n=4/5 ind. experiments). (C) Depiction of molecular interactions of a type I kinase inhibitor with a kinase domain (N and C lobe). Impact of Abemaciclib on indicated CDK6 kinase conformations (wt and p16INK4a binding deficient). 48 hr post transfection HEK293T cells expressing respective reporter constructs were treated with indicated inhibitors for 3 hr (1 μM) followed by RLuc PCA analyses (mean ± SEM, n=4 ind. experiments). (D) Bioluminescence measurement of PKAc wt and L206R KinCon reporters. HEK293T cells expressing the reporter were treated with 20 μM of Forskolin for 15 min followed by RLuc PCA analyses (mean ± SEM, n=4 ind. experiments). (E) Kinome tree displays kinases for which KinCon reporters have been generated (red dots). The blue squares highlight the kinases for

    Journal: eLife

    Article Title: Kinases in motion: impact of protein and small molecule interactions on kinase conformations

    doi: 10.7554/elife.94755

    Figure Lengend Snippet: Figure 5. Impact of small molecules and protein interactions on kinase activity conformations. (A+B) Depiction of molecular interactions of a type I 1/2 and type III kinase inhibitors with a kinase domain (N and C lobe). Impact of PLX8394, Cobimetinib and GSK547 on wild-type (wt) and mutated versions of BRAF, RIPK1, and MEK1 kinase conformation (KinCon) reporters. 48 hr post transfection HEK293T cells expressing respective reporter constructs were treated with indicated inhibitors for 1 hr (1 μM) followed by RLuc PCA analyses (mean ± SEM, n=4/5 ind. experiments). (C) Depiction of molecular interactions of a type I kinase inhibitor with a kinase domain (N and C lobe). Impact of Abemaciclib on indicated CDK6 kinase conformations (wt and p16INK4a binding deficient). 48 hr post transfection HEK293T cells expressing respective reporter constructs were treated with indicated inhibitors for 3 hr (1 μM) followed by RLuc PCA analyses (mean ± SEM, n=4 ind. experiments). (D) Bioluminescence measurement of PKAc wt and L206R KinCon reporters. HEK293T cells expressing the reporter were treated with 20 μM of Forskolin for 15 min followed by RLuc PCA analyses (mean ± SEM, n=4 ind. experiments). (E) Kinome tree displays kinases for which KinCon reporters have been generated (red dots). The blue squares highlight the kinases for

    Article Snippet: DOI: https://doi.org/10.7554/eLife.94755 20 of 31 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Transfected construct mouse pcDNA3.1CDK6- F[1] CDK6- PPI reporter Transfected construct mouse pcDNA3.1CDK6- R31C- F[1] CDK6- PPI reporter (R31C) Transfected construct mouse pcDNA3.1CDK4- F[1] CDK4- PPI reporter Transfected construct mouse pcDNA3.1CDK4- R24C- F[1] CDK4- PPI reporter (R24C) Transfected construct mouse pcDNA3.1p16INK4a- F[2] p16INK4a- PPI reporter Transfected construct mouse pcDNA3.1- p16INK4a- P40L- F[2] p16INK4a- PPI reporter (P40L) Transfected construct human pcDNA3.1F[1]-MEK1- F[2] MEK1- KinCon Transfected construct human pcDNA3.1- F[1]-MEK1- K57E- F[2] MEK- KinCon (K57E) Transfected construct human pcDNA3.1F[1]-PKAc- F[2] PKAc- KinCon Transfected construct human pcDNA3.1F[1]-PKAc- L206R- F[2] PKAc- KinCon (L206R) Transfected construct human pcDNA3.1MO25- 3xFlag MO25- 3xFlag Transfected construct human pcDNA3.1F[1]-BRAF- F[2] BRAF- KinCon Transfected construct human pcDNA3.1F[1]-BRAF- V600E- F[2] BRAF- KinCon (V600E) Transfected construct human pcDNA3.1F[1]-RIPK1- F[2] RIPK1- KinCon Transfected construct human pcDNA3.1F[1]-RIPK2- F[2] RIPK2- KinCon Transfected construct human pcDNA3.1F[1]-RIPK3- F[2] RIPK3- KinCon Antibody GAPDH Rabbit monoclonal Cell Signaling 2118 1:10000 Antibody AMPKα Rabbit monoclonal Cell Signaling 2532 1:1000 Antibody PhosphoAMPKα(Thr172) Rabbit monoclonal Cell Signaling 2535 1:1000 Antibody LKB1 Rabbit monoclonal Cell Signaling 3047 1:1000 Antibody Vinculin Rabbit monoclonal Cell Signaling 4650 1:1000 Antibody RIP XP Rabbit monoclonal Cell Signaling 3493 1:1000 Antibody RIP3 Rabbit monoclonal Cell Signaling 13526 1:1000 Antibody CDK6 Mouse monoclonal Cell Signaling 3136 1:1000 Continued Continued on next page Kugler, Schwaighofer et al. eLife 2024;13:RP94755.

    Techniques: Activity Assay, Transfection, Expressing, Construct, Binding Assay, Generated

    Figure 3. Effects of RAPA on the cell cycle progress of B16‑F10 cells. (A) Cell cycle distribution was assessed by flow cytometry. (B) Results of statistical analysis of cell rates at different time periods for each group in (A). (C) CDK1, cyclin D1, CDK4, CDK6, cyclin E1 and CDK2 protein expression levels were detected by western blotting. Relative expression of (D) CDK1, (E) Cyclin D1, (F) CDK4, (G) CDK6, (H) Cyclin E1 and (I) CDK2 proteins. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. RAPA 0 nM group. ns, not significant; RAPA, rapamycin.

    Journal: Oncology letters

    Article Title: Rapamycin inhibits B16 melanoma cell viability in vitro and in vivo by inducing autophagy and inhibiting the mTOR/p70‑S6k pathway.

    doi: 10.3892/ol.2024.14273

    Figure Lengend Snippet: Figure 3. Effects of RAPA on the cell cycle progress of B16‑F10 cells. (A) Cell cycle distribution was assessed by flow cytometry. (B) Results of statistical analysis of cell rates at different time periods for each group in (A). (C) CDK1, cyclin D1, CDK4, CDK6, cyclin E1 and CDK2 protein expression levels were detected by western blotting. Relative expression of (D) CDK1, (E) Cyclin D1, (F) CDK4, (G) CDK6, (H) Cyclin E1 and (I) CDK2 proteins. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. RAPA 0 nM group. ns, not significant; RAPA, rapamycin.

    Article Snippet: Mouse GAPDH monoclonal antibody (cat. no. 60004‐1‐Ig), mouse Beclin‐1 monoclonal antibody (cat. no. 66665‐1‐Ig), mouse caspase 3/p17/p19 monoclonal antibody (cat. no. 66470‐2‐Ig), mouse Bax monoclonal antibody (cat. no. 60267‐1‐Ig), mouse Bcl2 monoclonal antibody (cat. no. 68103‐1‐Ig), mouse cyclin D1 monoclonal antibody (cat. no. 60186‐1‐Ig), mouse CDK4 monoclonal anti‐ body (cat. no. 66950‐1‐Ig), mouse CDK6 monoclonal antibody (cat. no. 66278‐1‐Ig), mouse CDK2 monoclonal antibody (cat. no. 60312‐1‐Ig), rabbit cyclin E1 polyclonal antibody (cat. no. 11554‐1‐AP), rabbit microtubule‐associated protein light chain 3 (LC3) polyclonal antibody (cat. no. 14600‐1‐AP), goat anti‐rabbit IgG‐HRP (cat. no. SA00001‐2) and goat anti‐mouse IgG‐HRP (cat. no. SA00001‐1) antibodies were purchased from ProteinTech Group, Inc. Rabbit sequestosome 1/p62 (cat. no. AF5384), mTOR (cat. no. AF6308), p70 ribosomal S6 kinase (p70‐S6k; cat. no. AF6226), eukaryotic initiation factor 4E‐binding protein (4E‐BP1; cat. no. AF6432), phosphorylated (p)‐mTOR (cat. no. AF3308), p‐p70‐S6k (cat. no. AF3228) and p‐4E‐BP1 (cat. no. AF3830) polyclonal antibodies were purchased from Affinity Biosciences.

    Techniques: Flow Cytometry, Expressing, Western Blot